Laird Advances DNA Replication Process with New PCR Cycler
Laird’s new Evaluation Kit enables faster and more reliable results for DNA replication processes. This allows medical equipment manufacturers and solutions providers to offer their customers more reliable and accurate results in less time which allows for shortened drug development times and increased throughput in testing labs.
For example, when diagnosing hereditary diseases, identifying genetic fingerprints (used in forensic sciences and paternity testing), and detecting or diagnosing infectious diseases, Polymerase Chain Reaction (PCR) is often used to replicate the DNA being tested. This is an exacting process, often times requiring the rapid heating and cooling of the DNA sample based on a predefined series of temperature steps. Simple temperature controllers can often overshoot set temperature points when ramping at these fast rates. This can frequently lead to inaccurate test results.
"The market is evolving toward faster chemistries used in DNA amplification. Too many manufacturers are using simple temperature controllers that are not sophisticated enough to manage fast thermal responses," explains Anders Kottenauer, Senior Vice President of Laird's Engineered Thermal Systems business. He adds, "Use of our new PCR Cycler technology can improve test accuracy and ultimately lead to the detection of new pathogens and treatment solutions.”
The PCR Cycler Evaluation Kit, identified as model PC-24-TS-58, includes a programmable controller that utilizes an ultrafast thermal feedback loop and two control loops, one for the control side and one for the heat rejection side. These allow the controller to quickly and accurately adjust to the precise temperatures required throughout the process cycle without increasing test time or interfering with the results.
The controller incorporates alarm features and a port for SD flash memory cards to log temperatures and electric current throughout the duration of DNA amplification. This verifies that the device is operating effectively and that DNA has been driven to specified temperature set point ranges.
The evaluation kit includes a thermoelectric assembly, cycling controller and graphical interface.